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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF18 Polyclonal antibody

ZNF18 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17070-1-AP

Synonyms

HDSG1, KOX11, Zfp535, zinc finger protein 18, Zinc finger protein KOX11, ZKSCAN6, ZNF18, ZNF535

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF18 Polyclonal antibody (17070-1-AP)

    WB analysis using 17070-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17070-1-AP (ZNF18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZNF18 Polyclonal antibody (17070-1-AP)

    WB analysis of HepG2 using 17070-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17070-1-AP (ZNF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF18 Polyclonal antibody (17070-1-AP)

    IHC staining of human brain using 17070-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17070-1-AP (ZNF18 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF18 Polyclonal antibody (17070-1-AP)

    IHC staining of human brain using 17070-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17070-1-AP (ZNF18 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17070-1-AP targets ZNF18 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF18 fusion protein Ag10897 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 18
Calculated molecular weight 549 aa, 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC036096
Gene symbol ZNF18
Gene ID (NCBI) 7566
RRIDAB_2218591
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF18 antibody 17070-1-APDownload protocol
IHC protocol for ZNF18 antibody 17070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols