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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF184 Polyclonal antibody

ZNF184 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26100-1-AP

Synonyms

zinc finger protein 184, ZNF184

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZNF184 Polyclonal antibody (26100-1-AP)

    WB analysis of HepG2 using 26100-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26100-1-AP (ZNF184 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ZNF184 Polyclonal antibody (26100-1-AP)

    WB analysis of MCF-7 using 26100-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26100-1-AP (ZNF184 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZNF184 Polyclonal antibody (26100-1-AP)

    WB analysis of Jurkat using 26100-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26100-1-AP (ZNF184 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF184 Polyclonal antibody (26100-1-AP)

    IF Staining of HepG2 using 26100-1-AP

    Immunofluorescent analysis of HepG2 cells using 26100-1-AP (ZNF184 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26100-1-AP is ZNF184 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF184 fusion protein Ag23314 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 184
Calculated molecular weight 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC022992
Gene symbol ZNF184
Gene ID (NCBI) 7738
RRIDAB_2880376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF184 antibody 26100-1-APDownload protocol
IF protocol for ZNF184 antibody 26100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols