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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF189 Monoclonal antibody

ZNF189 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A4B4

Cat no : 68424-1-Ig

Synonyms

zinc finger protein 189, ZNF189

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF189 Monoclonal antibody (68424-1-Ig)

    WB analysis using 68424-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68424-1-Ig (ZNF189 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HeLa cells using ZNF189 Monoclonal antibody (68424-1-Ig)

    WB analysis of HeLa using 68424-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68424-1-Ig (ZNF189 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68424-1-Ig targets ZNF189 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ZNF189 fusion protein Ag33164 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 189
Observed molecular weight70-73 kDa
GenBank accession numberBC126120
Gene symbol ZNF189
Gene ID (NCBI) 7743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF189 antibody 68424-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols