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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF215 Polyclonal antibody

ZNF215 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25502-1-AP

Synonyms

BAZ 2, BAZ2, zinc finger protein 215, ZKSCAN11, ZNF215

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ZNF215 Polyclonal antibody (25502-1-AP)

    WB analysis of mouse brain using 25502-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25502-1-AP (ZNF215 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF215 Polyclonal antibody (25502-1-AP)

    IF Staining of HepG2 using 25502-1-AP

    Immunofluorescent analysis of HepG2 cells using 25502-1-AP (ZNF215 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25502-1-AP is ZNF215 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF215 fusion protein Ag22026 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 215
Calculated molecular weight 517 aa, 60 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC014538
Gene symbol ZNF215
Gene ID (NCBI) 7762
RRIDAB_2880105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF215 antibody 25502-1-APDownload protocol
IF protocol for ZNF215 antibody 25502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols