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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF217 Polyclonal antibody (24869-1-AP)

    WB analysis using 24869-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24869-1-AP (ZNF217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NCCIT cells using ZNF217 Polyclonal antibody (24869-1-AP)

    IP experiment of NCCIT using 24869-1-AP

    IP result of anti-ZNF217 (IP:24869-1-AP, 4ug; Detection:24869-1-AP 1:300) with NCCIT cell lysate 3200ug.

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ZNF217 Polyclonal antibody

ZNF217 Polyclonal Antibody for WB, IP, ELISA
Cat No. 24869-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

ZABC1, ZFP217, zinc finger protein 217, ZNF217

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, LNCaP cells, MDA-MB-231 cells
Positive IP detected inNCCIT cell

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24869-1-AP targets ZNF217 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF217 fusion protein Ag19468 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 217
Calculated molecular weight 1048 aa, 115 kDa
Observed molecular weight~130 kDa
GenBank accession numberBC113427
Gene Symbol ZNF217
Gene ID (NCBI) 7764
RRIDAB_3085760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75362
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF217 antibody 24869-1-APDownload protocol
IP protocol for ZNF217 antibody 24869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols