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  • Featured Product
  • KD/KO Validated

ZNF251 Polyclonal antibody

ZNF251 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25601-1-AP

Synonyms

zinc finger protein 251, ZNF251

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ZNF251 Polyclonal antibody (25601-1-AP)

    WB analysis of mouse testis using 25601-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25601-1-AP (ZNF251 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ZNF251 Polyclonal antibody (25601-1-AP)

    WB analysis of mouse skeletal muscle using 25601-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25601-1-AP (ZNF251 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZNF251 Polyclonal antibody (25601-1-AP)

    WB analysis of HepG2 using 25601-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25601-1-AP (ZNF251 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using ZNF251 Polyclonal antibody (25601-1-AP)

    WB analysis of SKOV-3 using 25601-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25601-1-AP (ZNF251 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using ZNF251 Polyclonal antibody (25601-1-AP)

    IF Staining of SKOV-3 using 25601-1-AP

    Immunofluorescent analysis of SKOV-3 cells using 25601-1-AP (ZNF251 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue, HepG2 cells, mouse skeletal muscle tissue, SKOV-3 cells
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25601-1-AP targets ZNF251 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF251 fusion protein Ag22347 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 251
Calculated molecular weight 671 aa, 76 kDa
Observed molecular weight 70-76 kDa
GenBank accession numberBC112137
Gene symbol ZNF251
Gene ID (NCBI) 90987
RRIDAB_2880154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF251 antibody 25601-1-APDownload protocol
IF protocol for ZNF251 antibody 25601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Res Sq

Haploinsufficiency of ZNF251 causes DNA-PKcs-dependent resistance to PARP inhibitors in BRCA1-mutated cancer cells

Authors - Huan Li
  • KD Validated
View Article