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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZNF253 Polyclonal antibody (25152-1-AP)

    WB analysis of HepG2 using 25152-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25152-1-AP (ZNF253 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ZNF253 Polyclonal antibody (25152-1-AP)

    WB analysis of SMMC-7721 using 25152-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25152-1-AP (ZNF253 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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ZNF253 Polyclonal antibody

ZNF253 Polyclonal Antibody for WB, ELISA
Cat No. 25152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BMZF 1, BMZF1, Bone marrow zinc finger 1, zinc finger protein 253, Zinc finger protein 411, ZNF253, ZNF411

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25152-1-AP targets ZNF253 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF253 fusion protein Ag18570 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 253
Calculated molecular weight 499 aa, 58 kDa
Observed molecular weight 58-65 kDa
GenBank accession numberBC125063
Gene Symbol ZNF253
Gene ID (NCBI) 56242
RRIDAB_2879927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75346
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF253, also named as Zinc finger protein 411 or BMZF1, is a 499 amino acid protein, which contains one KRAB domain and eleven C2H2-type zinc fingers. ZNF253 belongs to the krueppel C2H2-type zinc-finger protein family and is expressed in bone marrow and in monocytic and immature erythroid cell lines. ZNF253 may function as a transcription factor.

Protocols

Product Specific Protocols
WB protocol for ZNF253 antibody 25152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols