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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF263 Polyclonal antibody

ZNF263 Polyclonal Antibody for IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 21326-1-AP

Synonyms

ZKSCAN12, Zinc finger protein FPM315, Zinc finger protein 263, ZFP263, FPM315

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HepG2 cells using ZNF263 Polyclonal antibody (21326-1-AP)

    IP experiment of HepG2 using 21326-1-AP

    IP result of anti-ZNF263 (IP:21326-1-AP, 4ug; Detection:21326-1-AP 1:300) with HepG2 cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ZNF263 Polyclonal antibody (21326-1-AP)

    IF Staining of U2OS using 21326-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF263 antibody (21326-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive IP detected inHepG2 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

ChIPSee 1 publications below

Product Information

21326-1-AP targets ZNF263 in IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF263 fusion protein Ag15158 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 263
Calculated molecular weight 683 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC008805
Gene symbol ZNF263
Gene ID (NCBI) 10127
RRIDAB_2878841
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF263 is a transcriptional repressor and activator that contains an N-terminal scanning domain and nine C2H2 zinc finger domains at the C-terminus, as well as a kruppel association box (KRAB) domain (PMID: 10567577). ZNF263 has been reported to be involved in early human embryonic development, cardiac regeneration, and adipogenesis (PMID: 37065635).

Protocols

Product Specific Protocols
IF protocol for ZNF263 antibody 21326-1-APDownload protocol
IP protocol for ZNF263 antibody 21326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanChIP

Front Oncol

Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma.

Authors - Min-Yun Cai
View Article