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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ZNF277 Polyclonal antibody (25094-1-AP)

    WB analysis of PC-3 using 25094-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25094-1-AP (ZNF277 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZNF277 Polyclonal antibody (25094-1-AP)

    WB analysis of Jurkat using 25094-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25094-1-AP (ZNF277 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF277 Polyclonal antibody (25094-1-AP)

    IF Staining of HepG2 using 25094-1-AP

    Immunofluorescent analysis of HepG2 cells using 25094-1-AP (ZNF277 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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ZNF277 Polyclonal antibody

ZNF277 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25094-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NRIF4, zinc finger protein 277, ZNF277, ZNF277P

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25094-1-AP targets ZNF277 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF277 fusion protein Ag18979 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 277
Calculated molecular weight 450 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC020626
Gene Symbol ZNF277
Gene ID (NCBI) 11179
RRIDAB_2879893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRM2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF277 antibody 25094-1-APDownload protocol
IF protocol for ZNF277 antibody 25094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols