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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF280A Polyclonal antibody (31903-1-AP)

    WB analysis using 31903-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31903-1-AP (ZNF280A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZNF280A Polyclonal antibody (31903-1-AP)

    WB analysis using 31903-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31903-1-AP (ZNF280A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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ZNF280A Polyclonal antibody

ZNF280A Polyclonal Antibody for WB, ELISA
Cat No. 31903-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Zinc finger protein 636, Zinc finger protein 280A, Zinc Finger Protein 280, Suppressor of hairy wing homolog 1, SUHW1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inK-562 cells, mouse adrenal gland tissue, mouse kidney tissue, rat kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31903-1-AP targets ZNF280A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF280A fusion protein Ag35942 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 280A
Calculated molecular weight60 kDa
Observed molecular weight50 kDa
GenBank accession numberBC053901
Gene Symbol ZNF280A
Gene ID (NCBI) 129025
RRIDAB_3670138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP59817
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger proteins are the largest transcription factor family in the human genome and play a significant role in regulating and managing gene expression, including development, differentiation, metabolism, and autophagy (PMID: 18253864; 27411336). Zinc finger protein 280A (ZNF280A, also known as SUHW1, ZNF280, and ZNF636) is a member of the zinc-finger protein family, carrying two consecutive Cys2His2 zinc finger domains (PMID: 33414445). Cys2-His2 (C2H2) zinc finger domains (ZFs) were originally identified as DNA-binding domains, and uncharacterized domains are typically assumed to function in DNA binding (PMID: 18253864).

Protocols

Product Specific Protocols
WB protocol for ZNF280A antibody 31903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols