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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF281 Polyclonal antibody (24088-1-AP)

    WB analysis using 24088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24088-1-AP (ZNF281 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ZNF281 Polyclonal antibody (24088-1-AP)

    IF Staining of U2OS using 24088-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF281 antibody (24088-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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ZNF281 Polyclonal antibody

ZNF281 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24088-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GZP1, Transcription factor ZBP 99, ZBP 99, ZBP99, zinc finger protein 281, ZNF281, ZNP 99

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNCCIT cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24088-1-AP targets ZNF281 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF281 fusion protein Ag21355 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 281
Calculated molecular weight 895 aa, 97 kDa
Observed molecular weight110 kDa
GenBank accession numberBC060820
Gene Symbol ZNF281
Gene ID (NCBI) 23528
RRIDAB_3085722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9Y2X9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF281 antibody 24088-1-APDownload protocol
IF protocol for ZNF281 antibody 24088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols