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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF287 Polyclonal antibody

ZNF287 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22162-1-AP

Synonyms

zinc finger protein 287, ZKSCAN13, ZNF287

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ZNF287 Polyclonal antibody (22162-1-AP)

    WB analysis of A549 using 22162-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22162-1-AP (ZNF287 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZNF287 Polyclonal antibody (22162-1-AP)

    IHC staining of human kidney using 22162-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22162-1-AP (ZNF287 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZNF287 Polyclonal antibody (22162-1-AP)

    IHC staining of human kidney using 22162-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22162-1-AP (ZNF287 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22162-1-AP is ZNF287 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF287 fusion protein Ag17814 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 287
Calculated molecular weight 761 aa, 88 kDa
Observed molecular weight 88-100 kDa
GenBank accession numberBC113363
Gene symbol ZNF287
Gene ID (NCBI) 57336
RRIDAB_2879013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF287 antibody 22162-1-APDownload protocol
IHC protocol for ZNF287 antibody 22162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols