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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF326 Polyclonal antibody

ZNF326 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25147-1-AP

Synonyms

ZAN75, Zfp326, zinc finger protein 326, ZNF326

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZNF326 Polyclonal antibody (25147-1-AP)

    WB analysis of Jurkat using 25147-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ZNF326 Polyclonal antibody (25147-1-AP)

    WB analysis of MCF-7 using 25147-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using ZNF326 Polyclonal antibody (25147-1-AP)

    WB analysis of PC-3 using 25147-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF326 Polyclonal antibody (25147-1-AP)

    WB analysis of HeLa using 25147-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ZNF326 Polyclonal antibody (25147-1-AP)

    IHC staining of mouse liver using 25147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ZNF326 Polyclonal antibody (25147-1-AP)

    IHC staining of mouse liver using 25147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNF326 Polyclonal antibody (25147-1-AP)

    IF Staining of HeLa using 25147-1-AP

    Immunofluorescent analysis of HeLa cells using 25147-1-AP (ZNF326 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25147-1-AP is ZNF326 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF326 fusion protein Ag18433 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 326
Calculated molecular weight 582 aa, 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC090866
Gene symbol ZNF326
Gene ID (NCBI) 284695
RRIDAB_2879923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF326 antibody 25147-1-APDownload protocol
IHC protocol for ZNF326 antibody 25147-1-APDownload protocol
IF protocol for ZNF326 antibody 25147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Sci Rep

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Authors - S Prpar Mihevc
View Article
mouseWB

Toxicol Sci

LncRNA NR_030777 Alleviates Paraquat-induced Neurotoxicity by Regulating Zfp326 and Cpne5.

Authors - Hongyu Yang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tsimafei (Verified Customer) (08-07-2024)

Antibody detect the band of the correct size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U2OS
ZNF326 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:25147-1-AP)