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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF34 Polyclonal antibody, PBS Only

ZNF34 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20477-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF34 Polyclonal antibody (20477-1-AP)

    WB analysis of HeLa using 20477-1-AP (same clone as 20477-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20477-1-AP (ZNF34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20477-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZNF34 Polyclonal antibody (20477-1-AP)

    WB analysis of Jurkat using 20477-1-AP (same clone as 20477-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20477-1-AP (ZNF34 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20477-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ZNF34 Polyclonal antibody (20477-1-AP)

    WB analysis of HepG2 using 20477-1-AP (same clone as 20477-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20477-1-AP (ZNF34 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF34 Polyclonal antibody (20477-1-AP)

    IHC staining of human brain using 20477-1-AP (same clone as 20477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 20477-1-AP (ZNF34 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF34 Polyclonal antibody (20477-1-AP)

    IHC staining of human brain using 20477-1-AP (same clone as 20477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 20477-1-AP (ZNF34 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20477-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20477-1-PBS targets ZNF34 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF34 fusion protein Ag14346 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 34
Calculated molecular weight539aa,62 kDa; 560aa,64 kDa
Observed molecular weight 62-68 kDa
GenBank accession numberBC004480
Gene symbol ZNF34
Gene ID (NCBI) 80778
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ZNF34, also named as KOX32, contains twelve C2H2-type zinc fingers and one KRAB domain. It belongs to the krueppel C2H2-type zinc-finger protein family. This protein may be involved in transcriptional regulation.