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  • Featured Product
  • KD/KO Validated

ZNF346 Polyclonal antibody

ZNF346 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20794-1-AP

Synonyms

Zinc finger protein 346, Zfp346, JAZ

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ZNF346 Polyclonal antibody (20794-1-AP)

    WB analysis of human brain using 20794-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20794-1-AP (ZNF346 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ZNF346 Polyclonal antibody (20794-1-AP)

    WB analysis of human brain using 20794-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20794-1-AP (ZNF346 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF346 Polyclonal antibody (20794-1-AP)

    IHC staining of human testis using 20794-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20794-1-AP (ZNF346 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ZNF346 Polyclonal antibody (20794-1-AP)

    IHC staining of human placenta using 20794-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20794-1-AP (ZNF346 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF346 Polyclonal antibody (20794-1-AP)

    IHC staining of human testis using 20794-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20794-1-AP (ZNF346 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ZNF346 Polyclonal antibody (20794-1-AP)

    IF Staining of A549 using 20794-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF346 antibody (20794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20794-1-AP targets ZNF346 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF346 fusion protein Ag14585 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 346
Calculated molecular weight 294 aa, 33 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC007775
Gene symbol ZNF346
Gene ID (NCBI) 23567
RRIDAB_10695766
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF346 antibody 20794-1-APDownload protocol
IHC protocol for ZNF346 antibody 20794-1-APDownload protocol
IF protocol for ZNF346 antibody 20794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

ZNF385A and ZNF346 Serve as Prognostic Biomarkers Associated with an Inflamed Immunosuppressive Tumor Microenvironment in Hepatocellular Carcinoma

Authors - Qihang Peng
  • KD Validated
View Article