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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using ZNF350 Recombinant antibody (84121-5-RR)

    WB analysis of THP-1 using 84121-5-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84121-5-RR (ZNF350 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using ZNF350 Recombinant antibody (84121-5-RR)

    FC experiment of A431 using 84121-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ZNF350 Recombinant antibody (84121-5-RR, Clone:241225D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84121-5-RR

    Affinity and Kinetic Characterization of 84121-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84121-5-RR against Human ZNF350 were performed. The affinity constant is below 1 pM.

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ZNF350 Recombinant antibody

ZNF350 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84121-5-RR
Clone No.241225D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Zinc finger protein ZBRK1, zinc finger protein 350, ZFQR, ZBRK1, KRAB zinc finger protein ZFQR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84121-5-RR targets ZNF350 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZNF350 fusion protein Ag23982 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 350
Observed molecular weight60 kDa
GenBank accession numberBC009921
Gene Symbol ZNF350
Gene ID (NCBI) 59348
RRIDAB_3671683
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9GZX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF350 (Zinc finger protein 350), also known as zinc-finger and BRCA1-interacting protein with a Kruppel-associated box (KRAB) domain (ZBRK1), is involved in the development of several human tumor types, including breast, colon, and cervical carcinomas. ZNF350 has been identified as a transcriptional suppressor that can either form complexes with other proteins or play a direct transcriptional suppressive role in a single-factor form (PMID: 30613364). Overexpression of ZBRK1 has the potential to inhibit cancer cell migration and suppress metastasis activity suggesting that ZBRK1 may behave as a metastasis suppressor (PMID: 19996286).

Protocols

Product Specific Protocols
WB protocol for ZNF350 antibody 84121-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols