Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse brain tissue, HeLa cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| ChIP | See 1 publications below |
Product Information
14529-1-AP targets ZNF410 in WB, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZNF410 fusion protein Ag6028 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 410 |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 52-67 kDa |
| GenBank accession number | BC050683 |
| Gene Symbol | ZNF410 |
| Gene ID (NCBI) | 57862 |
| RRID | AB_2257520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZNF410 (Zinc Finger Protein 410) is a transcription factor involved in the regulation of gene expression, particularly in the context of fetal hemoglobin (HbF) repression and chromatin remodeling. This protein is highly conserved among mammals and plays a crucial role in erythroid cells. The study found that ZNF410 uniquely silences HbF by activating its sole target gene, CHD4, a known HbF repressor in erythroid cells. (PMID: 40158221)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF410 antibody 14529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression. | ||
EMBO J PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase. | ||
Mol Ther Development of a double shmiR lentivirus effectively targeting both BCL11A and ZNF410 for enhanced induction of fetal hemoglobin to treat β-hemoglobinopathies. | ||
Biomedicines Heteronemin Suppresses Lymphangiogenesis through ARF-1 and MMP-9/VE-Cadherin/Vimentin. | ||
Free Radic Biol Med Critical role of caveolin-1 in intestinal ischemia reperfusion by inhibiting protein kinase C βII |