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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using ZNF486 Polyclonal antibody (20599-1-AP)

    WB analysis of COLO 320 using 20599-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20599-1-AP (ZNF486 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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ZNF486 Polyclonal antibody

ZNF486 Polyclonal Antibody for WB, ELISA
Cat No. 20599-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HCG1999761, isoform CRA_b, KRBO2, zinc finger protein 486, ZNF486

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20599-1-AP targets ZNF486 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF486 fusion protein Ag14567 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 486
Calculated molecular weight 463 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC008936
Gene Symbol ZNF486
Gene ID (NCBI) 90649
RRIDAB_2878707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96H40
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF486 antibody 20599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols