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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF521 Polyclonal antibody (23716-1-AP)

    WB analysis using 23716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23716-1-AP (ZNF521 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ZNF521 Polyclonal antibody (23716-1-AP)

    IF Staining of A431 using 23716-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNF521 antibody (23716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ZNF521 Polyclonal antibody

ZNF521 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23716-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Zfp521, zinc finger protein 521, LYST interacting protein 3, LIP3, Evi3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHuH-7 cells, L02 cells, T2 cells, THP-1 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23716-1-AP targets ZNF521 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF521 fusion protein Ag20540 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 521
Calculated molecular weight 1311 aa, 148 kDa
Observed molecular weight190 kDa
GenBank accession numberBC113648
Gene Symbol ZNF521
Gene ID (NCBI) 25925
RRIDAB_3669415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96K83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger protein 521 (EHZF/ZNF521) is a transcription co-factor originally identified for its abundant and selective expression in early progenitors of the human hematopoietic system. In the hematopoietic system ZNF521 is highly expressed in stem and progenitor cells but not in more differentiated precursors or mature leukocytes (PMID: 23907569, 21593590).

Protocols

Product Specific Protocols
WB protocol for ZNF521 antibody 23716-1-APDownload protocol
IF protocol for ZNF521 antibody 23716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols