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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF557 Polyclonal antibody

ZNF557 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20500-1-AP

Synonyms

zinc finger protein 557, ZNF557

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZNF557 Polyclonal antibody (20500-1-AP)

    WB analysis of HepG2 using 20500-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20500-1-AP (ZNF557 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF557 Polyclonal antibody (20500-1-AP)

    WB analysis of HeLa using 20500-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20500-1-AP (ZNF557 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ZNF557 Polyclonal antibody (20500-1-AP)

    WB analysis of A549 using 20500-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 20500-1-AP (ZNF557 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of COLO 320 cells using ZNF557 Polyclonal antibody (20500-1-AP)

    WB analysis of COLO 320 using 20500-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20500-1-AP (ZNF557 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF557 Polyclonal antibody (20500-1-AP)

    IF Staining of HepG2 using 20500-1-AP

    Immunofluorescent analysis of HepG2 cells using 20500-1-AP (ZNF557 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, A549 cells, COLO 320 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20500-1-AP is ZNF557 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF557 fusion protein Ag14239 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 557
Calculated molecular weight 423 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC003020
Gene symbol ZNF557
Gene ID (NCBI) 79230
RRIDAB_2878695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF557 antibody 20500-1-APDownload protocol
IF protocol for ZNF557 antibody 20500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

J Virol

KRAB-ZFP repressors enforce quiescence of oncogenic human herpesviruses.

Authors - Xiaofan Li
View Article