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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ZNF571 Polyclonal antibody (25302-1-AP)

    WB analysis of MCF-7 using 25302-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25302-1-AP (ZNF571 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF571 Polyclonal antibody (25302-1-AP)

    WB analysis of HeLa using 25302-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25302-1-AP (ZNF571 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using ZNF571 Polyclonal antibody (25302-1-AP)

    IP experiment of MCF-7 using 25302-1-AP

    IP result of anti-ZNF571 (IP:25302-1-AP, 4ug; Detection:25302-1-AP 1:300) with MCF-7 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF571 Polyclonal antibody (25302-1-AP)

    IHC staining of human testis using 25302-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25302-1-AP (ZNF571 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF571 Polyclonal antibody (25302-1-AP)

    IHC staining of human testis using 25302-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25302-1-AP (ZNF571 Antibody) at dilution of 1:50 (under 10x lens).

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ZNF571 Polyclonal antibody

ZNF571 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 25302-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Synonyms

HSPC059, zinc finger protein 571, ZNF571

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25302-1-AP targets ZNF571 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF571 fusion protein Ag18151 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 571
Calculated molecular weight 609 aa, 71 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC114479
Gene Symbol ZNF571
Gene ID (NCBI) 51276
RRIDAB_2880019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3V5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF571 antibody 25302-1-APDownload protocol
IHC protocol for ZNF571 antibody 25302-1-APDownload protocol
IP protocol for ZNF571 antibody 25302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols