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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZNF586 Polyclonal antibody (25023-1-AP)

    WB analysis of HepG2 using 25023-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25023-1-AP (ZNF586 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using ZNF586 Polyclonal antibody (25023-1-AP)

    WB analysis of COLO 320 using 25023-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25023-1-AP (ZNF586 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZNF586 Polyclonal antibody (25023-1-AP)

    WB analysis of HepG2 using 25023-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25023-1-AP (ZNF586 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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ZNF586 Polyclonal antibody

ZNF586 Polyclonal Antibody for WB, ELISA
Cat No. 25023-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

zinc finger protein 586, ZNF586

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25023-1-AP targets ZNF586 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF586 fusion protein Ag21732 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 586
Calculated molecular weight 402 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC124558
Gene Symbol ZNF586
Gene ID (NCBI) 54807
RRIDAB_2879853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXT0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF586 antibody 25023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols