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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF618 Polyclonal antibody (31972-1-AP)

    WB analysis using 31972-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31972-1-AP (ZNF618 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ZNF618 Polyclonal antibody (31972-1-AP)

    IHC staining of human stomach cancer using 31972-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ZNF618 Polyclonal antibody (31972-1-AP)

    IHC staining of human cervical cancer using 31972-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ZNF618 Polyclonal antibody (31972-1-AP)

    IF Staining of A549 using 31972-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF618 antibody (31972-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ZNF618 Polyclonal antibody

ZNF618 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31972-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Zinc finger protein 618, KIAA1952

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, SH-SY5Y cells, Caki-2 cells
Positive IHC detected inhuman cervical cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31972-1-AP targets ZNF618 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF618 fusion protein Ag36487 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 618
Calculated molecular weight105kDa，954aa
Observed molecular weight100-113 kDa
GenBank accession numberNM_001318042.1
Gene Symbol ZNF618
Gene ID (NCBI) 114991
RRIDAB_3670163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5T7W0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells.

Protocols

Product Specific Protocols
WB protocol for ZNF618 antibody 31972-1-APDownload protocol
IHC protocol for ZNF618 antibody 31972-1-APDownload protocol
IF protocol for ZNF618 antibody 31972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols