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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF638 Polyclonal antibody (31300-1-AP)

    WB analysis using 31300-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31300-1-AP (ZNF638 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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ZNF638 Polyclonal antibody

ZNF638 Polyclonal Antibody for WB, ELISA
Cat No. 31300-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

CTCL associated antigen se33 1, DKFZp686P1231, Nuclear protein 220, ZFML, Zfp638, zinc finger protein 638, ZNF638

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31300-1-AP targets ZNF638 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF638 fusion protein Ag35296 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 638
Calculated molecular weight221kDa
Observed molecular weight 270-300 kDa
GenBank accession numberNM_014497.4
Gene Symbol ZNF638
Gene ID (NCBI) 27332
RRIDAB_3669937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ14966
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404).

Protocols

Product Specific Protocols
WB protocol for ZNF638 antibody 31300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols