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Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF645 Polyclonal antibody

ZNF645 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25600-1-AP

Synonyms

HAKAIL, zinc finger protein 645, ZNF645

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using ZNF645 Polyclonal antibody (25600-1-AP)

    WB analysis of human testis using 25600-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 25600-1-AP (ZNF645 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZNF645 Polyclonal antibody (25600-1-AP)

    IHC staining of human prostate cancer using 25600-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25600-1-AP (ZNF645 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZNF645 Polyclonal antibody (25600-1-AP)

    IHC staining of human prostate cancer using 25600-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25600-1-AP (ZNF645 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25600-1-AP is ZNF645 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF645 fusion protein Ag22346 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 645
Calculated molecular weight 425 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC126190
Gene symbol ZNF645
Gene ID (NCBI) 158506
RRIDAB_2880153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF645 antibody 25600-1-APDownload protocol
IHC protocol for ZNF645 antibody 25600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Mi (Verified Customer) (05-29-2023)

It works well in human white adipocytes, we get two bands, with the lower band at the predicted size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: adipocytes