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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF654 Polyclonal antibody

ZNF654 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24596-1-AP

Synonyms

Melanoma associated antigen, zinc finger protein 654, ZNF654

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using ZNF654 Polyclonal antibody (24596-1-AP)

    WB analysis of mouse ovary using 24596-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 24596-1-AP (ZNF654 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ZNF654 Polyclonal antibody (24596-1-AP)

    WB analysis of MCF-7 using 24596-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24596-1-AP (ZNF654 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using ZNF654 Polyclonal antibody (24596-1-AP)

    IHC staining of mouse ovary using 24596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24596-1-AP (ZNF654 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using ZNF654 Polyclonal antibody (24596-1-AP)

    IHC staining of mouse ovary using 24596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24596-1-AP (ZNF654 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF654 Polyclonal antibody (24596-1-AP)

    IF Staining of HepG2 using 24596-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF654 antibody (24596-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse ovary tissue, MCF-7 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24596-1-AP is ZNF654 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF654 fusion protein Ag20186 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 654
Calculated molecular weight 581 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC153004
Gene symbol ZNF654
Gene ID (NCBI) 55279
RRIDAB_2879630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF654 antibody 24596-1-APDownload protocol
IHC protocol for ZNF654 antibody 24596-1-APDownload protocol
IF protocol for ZNF654 antibody 24596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols