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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF74 Polyclonal antibody

ZNF74 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21843-1-AP

Synonyms

Cos52, hZNF7, Zfp520, zinc finger protein 74, ZNF520, ZNF74

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZNF74 Polyclonal antibody (21843-1-AP)

    WB analysis of Jurkat using 21843-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21843-1-AP (ZNF74 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ZNF74 Polyclonal antibody (21843-1-AP)

    WB analysis of human brain using 21843-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21843-1-AP (ZNF74 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF74 Polyclonal antibody (21843-1-AP)

    IF Staining of HepG2 using 21843-1-AP

    Immunofluorescent analysis of HepG2 cells, using ZNF74 antibody 21843-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inJurkat cells, human brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21843-1-AP is ZNF74 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF74 fusion protein Ag13581 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 74
Calculated molecular weight 644 aa, 72 kDa
Observed molecular weight 64-72 kDa
GenBank accession numberBC013395
Gene symbol ZNF74
Gene ID (NCBI) 7625
RRIDAB_10836937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF74 antibody 21843-1-APDownload protocol
IF protocol for ZNF74 antibody 21843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomolecules

5'-tRF-19-Q1Q89PJZ Suppresses the Proliferation and Metastasis of Pancreatic Cancer Cells via Regulating Hexokinase 1-Mediated Glycolysis

Authors - Wenpeng Cao
View Article