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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF740 Polyclonal antibody

ZNF740 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25411-1-AP

Synonyms

Zinc finger protein 740, Zfp740, TB7, OriLyt TD-element-binding protein 7

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ZNF740 Polyclonal antibody (25411-1-AP)

    WB analysis of mouse brain using 25411-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using ZNF740 Polyclonal antibody (25411-1-AP)

    WB analysis of PC-3 using 25411-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ZNF740 Polyclonal antibody (25411-1-AP)

    WB analysis of mouse liver using 25411-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ZNF740 Polyclonal antibody (25411-1-AP)

    WB analysis of mouse testis using 25411-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ZNF740 Polyclonal antibody (25411-1-AP)

    IP experiment of mouse brain using 25411-1-AP

    IP result of anti-ZNF740 (IP:25411-1-AP, 3ug; Detection:25411-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ZNF740 Polyclonal antibody (25411-1-AP)

    IHC staining of human ovary cancer using 25411-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25411-1-AP (ZNF740 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, PC-3 cells, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25411-1-AP targets ZNF740 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF740 fusion protein Ag22295 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 740
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight 22-30 kDa
GenBank accession numberBC053557
Gene symbol ZNF740
Gene ID (NCBI) 283337
RRIDAB_2880065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF740 antibody 25411-1-APDownload protocol
IHC protocol for ZNF740 antibody 25411-1-APDownload protocol
IP protocol for ZNF740 antibody 25411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator

Authors - Lixia Zhang
  • KD Validated
View Article