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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF8 Polyclonal antibody

ZNF8 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 17448-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF8 Polyclonal antibody (17448-1-AP)

    WB analysis using 17448-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17448-1-AP (ZNF8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ZNF8 Polyclonal antibody (17448-1-AP)

    IHC staining of mouse cerebellum using 17448-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17448-1-AP (ZNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using ZNF8 Polyclonal antibody (17448-1-AP)

    IF Staining of mouse cerebellum using 17448-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using ZNF8 Polyclonal antibody (17448-1-AP)

    IF Staining of mouse cerebellum using 17448-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17448-1-AP targets ZNF8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF8 fusion protein Ag11571 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 8
Calculated molecular weight 575 aa, 65 kDa
Observed molecular weight65 kDa
GenBank accession numberBC039323
Gene symbol ZNF8
Gene ID (NCBI) 7554
RRIDAB_3669275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF8 is a transcriptional repressor. It may modulate BMP and TGF-beta signal transduction, through its interaction with SMAD proteins.

Protocols

Product Specific Protocols
IHC protocol for ZNF8 antibody 17448-1-APDownload protocol
IF protocol for ZNF8 antibody 17448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols