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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNHIT2 Polyclonal antibody (16885-1-AP)

    WB analysis using 16885-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16885-1-AP (ZNHIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ZNHIT2 Polyclonal antibody (16885-1-AP)

    WB analysis of human brain using 16885-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16885-1-AP (ZNHIT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZNHIT2 Polyclonal antibody (16885-1-AP)

    WB analysis using 16885-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16885-1-AP (ZNHIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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ZNHIT2 Polyclonal antibody

ZNHIT2 Polyclonal Antibody for WB, ELISA
Cat No. 16885-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

C11orf5, FON, Protein FON, zinc finger, HIT type 2, ZNHIT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16885-1-AP targets ZNHIT2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNHIT2 fusion protein Ag10351 相同性解析による交差性が予測される生物種
Full Name zinc finger, HIT type 2
Calculated molecular weight 403 aa, 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC052240
Gene Symbol ZNHIT2
Gene ID (NCBI) 741
RRIDAB_2220389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNHIT2 belongs to a family of HIT-finger proteins characterized by the presence of a HIT domain, a roughly 50-residue zinc-binding domain containing conserved cysteine (Cys) and histidine (His) residues. ZNHIT2 is a cofactor of R2TP/PFDL that targets the U5 snRNP. ZNHIT2 is highly expressed in testis.

Protocols

Product Specific Protocols
WB protocol for ZNHIT2 antibody 16885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols