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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNHIT2 Recombinant antibody (84418-7-RR)

    WB analysis using 84418-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84418-7-RR (ZNHIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84418-7-RR

    Affinity and Kinetic Characterization of 84418-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84418-7-RR against Human ZNHIT2 were performed. The affinity constant is below 1 pM.

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ZNHIT2 Recombinant antibody

ZNHIT2 Recombinant Antibody for WB, ELISA
Cat No. 84418-7-RR
Clone No.241868B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZNHIT 2, Zinc finger HIT domain-containing protein 2, FON, C11orf5, 241868B10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293T cells, K-562 cells, HeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84418-7-RR targets ZNHIT2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZNHIT2 fusion protein Ag10351 相同性解析による交差性が予測される生物種
Full Name zinc finger, HIT type 2
Calculated molecular weight 403 aa, 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC052240
Gene Symbol ZNHIT2
Gene ID (NCBI) 741
RRIDAB_3671947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UHR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNHIT2 belongs to a family of HIT-finger proteins characterized by the presence of a HIT domain, a roughly 50-residue zinc-binding domain containing conserved cysteine (Cys) and histidine (His) residues. ZNHIT2 is a cofactor of R2TP/PFDL that targets the U5 snRNP. ZNHIT2 is highly expressed in testis.

Protocols

Product Specific Protocols
WB protocol for ZNHIT2 antibody 84418-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols