Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat brain tissue, mouse brain tissue, mouse testis tissue, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
20200-1-AP targets ZNRF2-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | zinc and ring finger 2 |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | NM_147128 |
| Gene Symbol | ZNRF2 |
| Gene ID (NCBI) | 223082 |
| RRID | AB_2878657 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NHG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZNRF2 also named as RNF202, may play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. The antibody is specific to ZNRF2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNRF2-Specific antibody 20200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Neurol ZNRF2 attenuates focal cerebral ischemia/reperfusion injury in rats by inhibiting mTORC1-mediated autophagy. | ||
Hum Cell ZNRF2 as an oncogene is transcriptionally regulated by CREB1 in breast cancer models | ||
J Cell Mol Med A pancancer analysis of the oncogenic role of ZNRF2 in human tumours
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