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CoraLite® Plus 488-conjugated ZO-1 Monoclonal antibody
ZO-1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, canine
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G4A1
Cat no : CL488-66452
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66452 targets ZO-1 in IF/ICC applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ZO-1 fusion protein Ag16454 相同性解析による交差性が予測される生物種 |
| Full Name | tight junction protein 1 (zona occludens 1) |
| Calculated molecular weight | 1748 aa, 195 kDa |
| Observed molecular weight | 230 kDa |
| GenBank accession number | BC111712 |
| Gene symbol | ZO-1 |
| Gene ID (NCBI) | 7082 |
| RRID | AB_2919329 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ZO-1 antibody CL488-66452 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
