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  • Featured Product
  • KD/KO Validated

ZO-2 Monoclonal antibody

ZO-2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D2E5

Cat no : 67387-1-Ig

Synonyms

Tight junction protein 2, Tight junction protein ZO 2, TJP2, X104, ZO 2, ZO2, ZO-2, Zona occludens protein 2, Zonula occludens protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZO-2 Monoclonal antibody (67387-1-Ig)

    WB analysis of HeLa using 67387-1-Ig

    WB result of ZO-2 antibody (67387-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZO-2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ZO-2 Monoclonal antibody (67387-1-Ig)

    WB analysis using 67387-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67387-1-Ig (ZO-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, LNCaP cells, PC-3 cells, HepG2 cells, HEK-293 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67387-1-Ig targets ZO-2 in WB, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZO-2 fusion protein Ag13577 相同性解析による交差性が予測される生物種
Full Name tight junction protein 2 (zona occludens 2)
Calculated molecular weight 134 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC027592
Gene symbol TJP2
Gene ID (NCBI) 9414
RRIDAB_2882631
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZO-2 antibody 67387-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols