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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZO-2 Recombinant antibody

ZO-2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230474H8

Cat no : 83111-4-RR

Synonyms

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZO-2 Recombinant antibody (83111-4-RR)

    WB analysis using 83111-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using ZO-2 Recombinant antibody (83111-4-RR)

    WB analysis of C6 using 83111-4-RR

    C6 cells were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ZO-2 Recombinant antibody (83111-4-RR)

    IF Staining of MCF-7 using 83111-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-2 antibody (83111-4-RR, Clone: 230474H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using ZO-2 Recombinant antibody (83111-4-RR)

    FC experiment of A431 using 83111-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ZO-2 Recombinant antibody (83111-4-RR, Clone:230474H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of U2OS cells using ZO-2 Recombinant antibody (83111-4-RR)

    FC experiment of U2OS using 83111-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug ZO-2 Recombinant antibody (83111-4-RR, Clone:230474H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83111-4-RR

    Affinity and Kinetic Characterization of 83111-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83111-4-RR against Human XRN2 were performed. The affinity constant is 80.7 pM.

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Tested Applications

Positive WB detected inHEK-293 cells, C6 cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83111-4-RR is ZO-2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZO-2 fusion protein Ag13534 相同性解析による交差性が予測される生物種
Full Name tight junction protein 2 (zona occludens 2)
Calculated molecular weight 134 kDa
Observed molecular weight150 kDa
GenBank accession numberBC027592
Gene symbol TJP2
Gene ID (NCBI) 9414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

Protocols

Product Specific Protocols
WB protocol for ZO-2 antibody 83111-4-RRDownload protocol
IF protocol for ZO-2 antibody 83111-4-RRDownload protocol
FC protocol for ZO-2 antibody 83111-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols