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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZO1 Recombinant antibody, PBS Only (Capture)

ZO1 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240150A1

Cat no : 82870-2-PBS

Synonyms

TJP1, ZO-1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00349-4

    Sandwich ELISA standard curve of MP00349-4

    Sandwich ELISA standard curve of MP00349-4, Human ZO-1 Recombinant Matched Antibody Pair - PBS only. 82870-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33182. 82870-9-PBS was HRP conjugated as the detection antibody. Range: 125-8000 pg/mL.

  • Sample test of MP00349-4

    Sample test of MP00349-4

    Serum of nine individual healthy human donors was measured. The ZO-1 concentration of detected samples was determined to be 352.4 pg/mL with a range of 83.4 - 1,272.8 pg/mL.

  • Sample test of MP00349-4

    Sample test of MP00349-4

    The mean ZO-1 concentration was determined to be 7.5 ng/mL in bEnd.3 cell extract based on a 1.8 mg/mL extract load.

  • Cytometric bead array standard curve of MP00349-1

    Cytometric bead array standard curve of MP00349-1

    Cytometric bead array standard curve of MP00349-1, ZO-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82870-2-PBS. Detection antibody: 82870-5-PBS. Standard: Ag33182. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00349-2

    Cytometric bead array standard curve of MP00349-2

    Cytometric bead array standard curve of MP00349-2, ZO-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82870-2-PBS. Detection antibody: 82870-3-PBS. Standard: Ag33182. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00349-3

    Cytometric bead array standard curve of MP00349-3

    Cytometric bead array standard curve of MP00349-3, ZO-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82870-2-PBS. Detection antibody: 82870-4-PBS. Standard: Ag33182. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 82870-2-PBS

    Affinity and Kinetic Characterization of 82870-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 82870-2-PBS against Human ZO1 was performed. The affinity constant is 1.05 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82870-2-PBS targets ZO1 as part of a matched antibody pair:

MP00349-1: 82870-2-PBS capture and 82870-5-PBS detection (validated in Cytometric bead array)

MP00349-2: 82870-2-PBS capture and 82870-3-PBS detection (validated in Cytometric bead array)

MP00349-3: 82870-2-PBS capture and 82870-4-PBS detection (validated in Cytometric bead array)

MP00349-4: 82870-2-PBS capture and 82870-9-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZO1 fusion protein Ag33182 相同性解析による交差性が予測される生物種
Full Name tight junction protein 1 (zona occludens 1)
Calculated molecular weight 1748 aa, 195 kDa
GenBank accession numberBC111712
Gene symbol ZO-1
Gene ID (NCBI) 7082
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.