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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZO-1 Recombinant antibody (82870-7-RR)

    WB analysis using 82870-7-RR (same clone as 82870-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82870-7-RR (ZO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZO-1 Recombinant antibody (82870-7-RR)

    WB analysis using 82870-7-RR (same clone as 82870-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82870-7-RR (ZO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ZO-1 Recombinant antibody (82870-7-RR)

    IP experiment of HEK-293 using 82870-7-RR (same clone as 82870-7-PBS)

    IP result of anti-ZO-1 (IP:82870-7-RR, 4ug; Detection:82870-7-RR 1:4000) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ZO-1 Recombinant antibody (82870-7-RR)

    IHC staining of mouse kidney using 82870-7-RR (same clone as 82870-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ZO-1 Recombinant antibody (82870-7-RR)

    IF Staining of MCF-7 using 82870-7-RR (same clone as 82870-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ZO-1 Recombinant antibody (82870-7-RR)

    IF Staining of U2OS using 82870-7-RR (same clone as 82870-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ZO-1 Recombinant antibody (82870-7-RR)

    IF Staining of U2OS using 82870-7-RR (same clone as 82870-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using ZO-1 Recombinant antibody (82870-7-RR)

    IF Staining of MDCK using 82870-7-RR (same clone as 82870-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using ZO-1 Recombinant antibody (82870-7-RR)

    FC experiment of MCF-7 using 82870-7-RR (same clone as 82870-7-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZO-1 Recombinant antibody (82870-7-RR, Clone:240150D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82870-7-RR

    Affinity and Kinetic Characterization of 82870-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82870-7-RR against Human ZO-1 were performed. The affinity constant is 16.2 nM.

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ZO-1 Recombinant antibody, PBS Only

ZO-1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 82870-7-PBS
Clone No.240150D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82870-7-PBS targets ZO-1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZO-1 fusion protein Ag33182 相同性解析による交差性が予測される生物種
Full Name tight junction protein 1 (zona occludens 1)
Calculated molecular weight 1748 aa, 195 kDa
Observed molecular weight 230 kDa
GenBank accession numberBC111712
Gene Symbol ZO-1
Gene ID (NCBI) 7082
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ07157
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).