Validation Data Gallery
Tested Applications
| Positive WB detected in | Caco-2 cells, HEK-293 cells, HT-29 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | rat colon tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00002 targets ZO-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | tight junction protein 1 (zona occludens 1) |
| Calculated molecular weight | 1748 aa, 195 kDa |
| Observed molecular weight | 230 kDa |
| GenBank accession number | BC111712 |
| Gene Symbol | ZO-1 |
| Gene ID (NCBI) | 7082 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | Q07157 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tight junction (or zonula occludens) forms the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZO-1 antibody RMX00002 | Download protocol |
| IHC protocol for ZO-1 antibody RMX00002 | Download protocol |
| IF protocol for ZO-1 antibody RMX00002 | Download protocol |
| IP protocol for ZO-1 antibody RMX00002 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |