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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZSCAN29 Polyclonal antibody

ZSCAN29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21630-1-AP

Synonyms

Zfp690, Zinc finger protein 690, ZNF690, ZSCAN29

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZSCAN29 Polyclonal antibody (21630-1-AP)

    WB analysis using 21630-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21630-1-AP (ZSCAN29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using ZSCAN29 Polyclonal antibody (21630-1-AP)

    IHC staining of human colon using 21630-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21630-1-AP (ZSCAN29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ZSCAN29 Polyclonal antibody (21630-1-AP)

    IHC staining of human colon using 21630-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21630-1-AP (ZSCAN29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21630-1-AP targets ZSCAN29 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZSCAN29 fusion protein Ag16239 相同性解析による交差性が予測される生物種
Full Name zinc finger and SCAN domain containing 29
Calculated molecular weight 852 aa, 97 kDa
Observed molecular weight96-97 kDa
GenBank accession numberBC109271
Gene symbol ZSCAN29
Gene ID (NCBI) 146050
RRIDAB_3085664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZSCAN29 (zinc finger and SCAN domain containing 29), also named as ZNF690 or Zfp690. It is predicted to be located in nucleus and ubiquitous expression in testis and lymph node. The calculated molecular weight of ZSCAN29 is 96 kDa. The genes ZSCAN29 (associated with five disorders: SCZ, BIP, MD, ASD and ADHD) and ZSCAN31 (associated with three disorders: SCZ, BIP and MD) encode zing finger transcription factor proteins that belong to the krueppel C2H2-type zinc-finger protein family. Zinc finger proteins are essential in multiple regulatory mechanisms and some have been associated with psychiatric disorders as reviewed by Squassina et al., 2019 (PMID: 34637873). It also enables sequence-specific double-stranded DNA binding activity and be predicted to be involved in regulation of transcription by RNA polymerase II.

Protocols

Product Specific Protocols
WB protocol for ZSCAN29 antibody 21630-1-APDownload protocol
IHC protocol for ZSCAN29 antibody 21630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols