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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZSCAN5A Polyclonal antibody

ZSCAN5A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19926-1-AP

Synonyms

Zinc finger protein 495, ZNF495, ZSCAN5, ZSCAN5A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZSCAN5A Polyclonal antibody (19926-1-AP)

    WB analysis using 19926-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19926-1-AP (ZSCAN5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZSCAN5A Polyclonal antibody (19926-1-AP)

    IHC staining of human testis using 19926-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19926-1-AP (ZSCAN5A antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZSCAN5A Polyclonal antibody (19926-1-AP)

    IHC staining of human testis using 19926-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19926-1-AP (ZSCAN5A antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19926-1-AP targets ZSCAN5A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZSCAN5A fusion protein Ag13898 相同性解析による交差性が予測される生物種
Full Name zinc finger and SCAN domain containing 5A
Calculated molecular weight 496 aa, 56 kDa
GenBank accession numberBC002636
Gene symbol ZSCAN5A
Gene ID (NCBI) 79149
RRIDAB_2878624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger and SCAN domain-containing protein 5A (ZSCAN5A), also known as ZNF495 or ZSCAN5, belongs to the ZSCAN family of transcription factors. ZSCAN transcription factors contain two major domains, the zinc finger domain and the SCAN box. ZSCAN5A has two isoforms and is predicted to be located in the nucleus, it is also predicted to be involved in the regulation of transcription by RNA polymerase II and may be involved in angiogenesis, cell apoptosis, differentiation, migration and invasion, proliferation (PMID: 31218096).

Protocols

Product Specific Protocols
WB protocol for ZSCAN5A antibody 19926-1-APDownload protocol
IHC protocol for ZSCAN5A antibody 19926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols