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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Zinc Alpha 2 Glycoprotein Monoclonal antibody (66178-1-Ig)

    WB analysis of human plasma using 66178-1-Ig (same clone as 66178-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66178-1-Ig (AZGP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66178-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Zinc Alpha 2 Glycoprotein Monoclonal antibody (66178-1-Ig)

    IHC staining of human breast cancer using 66178-1-Ig (same clone as 66178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66178-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Zinc Alpha 2 Glycoprotein Monoclonal antibody (66178-1-Ig)

    IHC staining of human breast cancer using 66178-1-Ig (same clone as 66178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66178-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Zinc Alpha 2 Glycoprotein Monoclonal antibody (66178-1-Ig)

    IHC staining of human prostate cancer using 66178-1-Ig (same clone as 66178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66178-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Zinc Alpha 2 Glycoprotein Monoclonal antibody (66178-1-Ig)

    IHC staining of human prostate cancer using 66178-1-Ig (same clone as 66178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66178-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using Zinc Alpha 2 Glycoprotein Monoclonal antibody (66178-1-Ig)

    IHC staining of human prostate hyperplasia using 66178-1-Ig (same clone as 66178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66178-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using Zinc Alpha 2 Glycoprotein Monoclonal antibody (66178-1-Ig)

    IHC staining of human prostate hyperplasia using 66178-1-Ig (same clone as 66178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66178-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Zinc Alpha 2 Glycoprotein Monoclonal antibody (66178-1-Ig)

    IF Staining of human breast cancer using 66178-1-Ig (same clone as 66178-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Zinc Alpha 2 Glycoprotein antibody (66178-1-Ig, Clone: 4B8A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66178-1-PBS in a different storage buffer formulation.

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Zinc Alpha 2 Glycoprotein Monoclonal antibody, PBS Only

Zinc Alpha 2 Glycoprotein Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66178-1-PBS
Clone No.4B8A4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AZGP1, ZA2G, ZAG, Zinc alpha 2 glycoprotein, Zinc-alpha-2-glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66178-1-PBS targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Zinc Alpha 2 Glycoprotein fusion protein Ag5661 相同性解析による交差性が予測される生物種
Full Name alpha-2-glycoprotein 1, zinc-binding
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC033830
Gene Symbol Zinc Alpha 2 Glycoprotein
Gene ID (NCBI) 563
RRIDAB_2881573
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25311
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Zinc-alpha-2-glycoprotein (AZGP1), a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract, and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).