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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Zyxin Monoclonal antibody, PBS Only

Zyxin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D7E7

Cat no : 60254-1-PBS

Synonyms

ESP 2, HED 2, ZYX, zyxin, Zyxin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Zyxin Monoclonal antibody (60254-1-Ig)

    WB analysis of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Zyxin Monoclonal antibody (60254-1-Ig)

    WB analysis of MCF-7 using 60254-1-Ig (same clone as 60254-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using Zyxin Monoclonal antibody (60254-1-Ig)

    WB analysis of A431 using 60254-1-Ig (same clone as 60254-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human testis using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human testis using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human breast cancer using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human breast cancer using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human cervical cancer using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human cervical cancer using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human placenta using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Zyxin Monoclonal antibody (60254-1-Ig)

    IHC staining of human placenta using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Zyxin Monoclonal antibody (60254-1-Ig)

    IF Staining of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60254-1-Ig (Zyxin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60254-1-PBS targets Zyxin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Zyxin fusion protein Ag0419 相同性解析による交差性が予測される生物種
Full Name zyxin
Calculated molecular weight 80 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC008743
Gene symbol Zyxin
Gene ID (NCBI) 7791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.