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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ChromoTek anti-IL6 recombinant VHH, for 1x Cys conjugation

IL6Cys1 is an unconjugated recombinant anti IL6 Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Cat no : IL6Cys1

Synonyms

IL-6 VHH, IL-6 single domain antibody (sdAb), Interleukin 6, BSF-2, CDF

Validation Data Gallery

  • BLI analysis of the interaction between varying concentrations of anti-IL-6 VHH and immobilised, biotinylated HumanKine IL-6 (HZ-1019). Fit indicates fitting of data to a 1:1 binding model.

    BLI analysis of the interaction between varying concentrations of anti-IL-6 VHH and immobilised, biotinylated HumanKine IL-6 (HZ-1019). Fit indicates fitting of data to a 1:1 binding model.

Product Information

IL6Cys1 is an unconjugated recombinant anti IL6 Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

ApplicationsConjugation
ReactivityHuman
ConjugateUnconjugated
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity3 nM
Molecular Weight13.7 kDa
FormLiquid
RRIDAB_3665393
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 0.09% sodium azide
Storage ConditionStore at -20°C
Shippingdry ice
BackgroundInterleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.