Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, HEK-293T cells, U-251 cells, mouse brain tissue, HeLa cells, rat brain tissue, MDA-MB-231 cells |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
27320-1-AP targets APP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | APP fusion protein Ag26299 相同性解析による交差性が予測される生物種 |
| Full Name | amyloid beta (A4) precursor protein |
| Calculated molecular weight | 87 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC004369 |
| Gene Symbol | APP |
| Gene ID (NCBI) | 351 |
| RRID | AB_2880842 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05067 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. APP can be cleaved into several chains, this antibody could recognize N-terminal fragment of APP (N-APP).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for APP antibody 27320-1-AP | Download protocol |
| IHC protocol for APP antibody 27320-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Alzheimers Res Ther Krüppel-like factor 5 accelerates the pathogenesis of Alzheimer's disease via BACE1-mediated APP processing. | ||
Brain Res Investıgatıon of the protectıve effect of long-term exercıse on molecular pathways and behavıours ın scopolamıne ınduced alzheımer's dısease-lıke condıtıon |