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  • Featured Product
  • KD/KO Validated

c-MYC Recombinant antibody, PBS Only

c-MYC Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B17

Cat no : 80845-1-PBS

Synonyms

MYC, MRTL, Class E basic helix-loop-helix protein 39, c Myc, bHLHe39

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using c-MYC Recombinant antibody (80845-1-RR)

    WB analysis using 80845-1-RR (same clone as 80845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80845-1-RR (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using c-MYC Recombinant antibody (80845-1-RR)

    WB analysis of A549 using 80845-1-RR (same clone as 80845-1-PBS)

    WB result of MYC antibody (80845-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYC transfected A549 cells. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using c-MYC Recombinant antibody (80845-1-RR)

    IHC staining of mouse brain using 80845-1-RR (same clone as 80845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using c-MYC Recombinant antibody (80845-1-RR)

    IF Staining of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using c-MYC Recombinant antibody (80845-1-RR)

    IF Staining of HEK-293 using 80845-1-RR (same clone as 80845-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using c-MYC Recombinant antibody (80845-1-RR)

    IF Staining of U2OS using 80845-1-RR (same clone as 80845-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80845-1-RR

    Affinity and Kinetic Characterization of 80845-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80845-1-RR against Human c-MYC were performed. The affinity constant is 2.03 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80845-1-PBS targets c-MYC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen c-MYC fusion protein Ag1263 相同性解析による交差性が予測される生物種
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight 49 kDa
Observed molecular weight50-65 kDa
GenBank accession numberBC000141
Gene symbol MYC
Gene ID (NCBI) 4609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.