c-SRC Polyclonal antibody
c-SRC Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, hamster, human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25978-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, CHO cells, COLO 320 cells, Jurkat cells, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
25978-1-AP targets c-SRC in WB, IHC, ELISA applications and shows reactivity with mouse, hamster, human samples.
| Tested Reactivity | mouse, hamster, human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | c-SRC fusion protein Ag22415 相同性解析による交差性が予測される生物種 |
| Full Name | v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC011566 |
| Gene symbol | SRC |
| Gene ID (NCBI) | 6714 |
| RRID | AB_2880316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for c-SRC antibody 25978-1-AP | Download protocol |
| IHC protocol for c-SRC antibody 25978-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci Design and screening of a novel neuropilin-1 targeted penetrating peptide for anti-angiogenic therapy in glioma. | ||
J Transl Med Evodiamine inhibits proliferation and induces apoptosis of nasopharyngeal carcinoma cells via the SRC/ERBB2-mediated MAPK/ERK signaling pathway | ||
Sci Adv Matrix-bound nanovesicles alleviate particulate-induced periprosthetic osteolysis | ||
Front Immunol In silico identification and verification of Tanshinone IIA-related prognostic genes in hepatocellular carcinoma | ||
