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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using cGAS Recombinant antibody (82869-1-RR)

    WB analysis using 82869-1-RR (same clone as 82869-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82869-1-RR (cGAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HAP1 cells using cGAS Recombinant antibody (82869-1-RR)

    WB analysis of HAP1 using 82869-1-RR (same clone as 82869-1-PBS)

    WB result of cGAS antibody (82869-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and cGAS knockout HAP1 cells. This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using cGAS Recombinant antibody (82869-1-RR)

    IF Staining of A431 using 82869-1-RR (same clone as 82869-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using cGAS Recombinant antibody (82869-1-RR)

    IF Staining of A431 using 82869-1-RR (same clone as 82869-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using cGAS Recombinant antibody (82869-1-RR)

    FC experiment of A431 using 82869-1-RR (same clone as 82869-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug cGAS Recombinant antibody (82869-1-RR, Clone:1H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82869-1-RR

    Affinity and Kinetic Characterization of 82869-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82869-1-RR against Human cGAS were performed. The affinity constant is 0.124 nM.

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  • Featured Product
  • KD/KO Validated

cGAS Recombinant antibody, PBS Only

cGAS Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82869-1-PBS
Clone No.1H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2'3'-cGAMP synthase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82869-1-PBS targets cGAS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen cGAS fusion protein Ag23455 相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 150
Calculated molecular weight 496 aa, 54 kDa
Observed molecular weight62 kDa
GenBank accession numberBC113608
Gene Symbol cGAS
Gene ID (NCBI) 115004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N884
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.