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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

citrate synthase Monoclonal antibody, PBS Only

citrate synthase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9G6

Cat no : 67784-1-PBS

Synonyms

citrate synthase, CS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using citrate synthase Monoclonal antibody (67784-1-Ig)

    WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67784-1-Ig (citrate synthase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using citrate synthase Monoclonal antibody (67784-1-Ig)

    WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67784-1-Ig (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using citrate synthase Monoclonal antibody (67784-1-Ig)

    IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using citrate synthase Monoclonal antibody (67784-1-Ig)

    IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using citrate synthase Monoclonal antibody (67784-1-Ig)

    IF Staining of MCF-7 using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using citrate synthase Monoclonal antibody (67784-1-Ig)

    IF Staining of HepG2 using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67784-1-PBS targets citrate synthase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen citrate synthase fusion protein Ag9255 相同性解析による交差性が予測される生物種
Full Name citrate synthase
Calculated molecular weight 466 aa, 52 kDa
GenBank accession numberBC010106
Gene symbol Citrate Synthase
Gene ID (NCBI) 1431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.