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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using cyclin B1 Monoclonal antibody (67686-1-Ig)

    WB analysis using 67686-1-Ig (same clone as 67686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67686-1-Ig (cyclin B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    WB analysis of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

    WB result of cyclin B1 antibody (67686-1-Ig; 1:3200; incubated at room temperature for 1.5 hours) with sh-Control and sh-cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IHC staining of human tonsillitis using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IHC staining of human tonsillitis using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IHC staining of rat skin using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IF Staining of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:650 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IF Staining of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IF Staining of HT-29 using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:650 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    FC experiment of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug cyclin B1 Monoclonal antibody (67686-1-Ig, Clone:1F3G11)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

cyclin B1 Monoclonal antibody, PBS Only

cyclin B1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67686-1-PBS
Clone No.1F3G11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67686-1-PBS targets cyclin B1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen cyclin B1 fusion protein Ag29444 相同性解析による交差性が予測される生物種
Full Name cyclin B1
Calculated molecular weight 48 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC006510
Gene Symbol Cyclin B1
Gene ID (NCBI) 891
RRIDAB_2882879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14635
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.