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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Dynamitin (69-376 AA) Polyclonal antibody

Dynamitin (69-376 AA) Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10030-2-AP

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, Dynamitin (69-376 AA), p50 dynamitin, RBP50

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Dynamitin (69-376 AA) Polyclonal antibody (10030-2-AP)

    WB analysis of mouse brain using 10030-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10030-2-AP (Dynamitin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Dynamitin (69-376 AA) Polyclonal antibody (10030-2-AP)

    WB analysis of human brain using 10030-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10030-2-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Dynamitin (69-376 AA) Polyclonal antibody (10030-2-AP)

    IP experiment of mouse brain using 10030-2-AP

    IP result of anti-Dynamitin (69-376 AA) (IP:10030-2-AP, 4ug; Detection:10030-2-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Dynamitin (69-376 AA) Polyclonal antibody (10030-2-AP)

    IHC staining of human gliomas using 10030-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Dynamitin (69-376 AA) Polyclonal antibody (10030-2-AP)

    IHC staining of human gliomas using 10030-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Dynamitin (69-376 AA) Polyclonal antibody (10030-2-AP)

    IHC staining of human lymphoma using 10030-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Dynamitin (69-376 AA) Polyclonal antibody (10030-2-AP)

    IHC staining of human lymphoma using 10030-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10030-2-AP targets Dynamitin (69-376 AA) in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name dynactin 2 (p50)
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC000718
Gene symbol Dynamitin
Gene ID (NCBI) 10540
RRIDAB_513348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the internal region (69-376aa) of full length p50 dynamitin.

Protocols

Product Specific Protocols
WB protocol for Dynamitin (69-376 AA) antibody 10030-2-APDownload protocol
IHC protocol for Dynamitin (69-376 AA) antibody 10030-2-APDownload protocol
IP protocol for Dynamitin (69-376 AA) antibody 10030-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols